A Study of The Molten Globule State of Cytochrome C by Viscometry

Authors

  • M. Stupák Department of Medicinal and Clinical Biochemistry and Labmed, Co., Faculty of Medicine, Košice, Slovakia
  • M. Antalík Department of Biochemistry, Faculty of Natural Science, P. J. Šafárik University, Košice, Slovakia | Depatment of Biophysics, Institute of Experimental Physics of Slovak Acadamy of Sciences, Košice, Slovakia

Keywords:

molten globular, cytochrome c, viscometer

Abstract

Combination of viscometry and CD spectrometry made it possible to suggest the existence of a new conformational state of cytochrome c at high temperatures and ionic strengths. The molten globule state of cytochrome c was found at acidic pH in the presence of high concentrations of a salt at 20 °C. A low-cooperative conformational change of the cytochrome c to the unfolded state was observed with increasing temperature. Viscometry is a suitable tool for studies of similar processes in biomacromolecules.

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Published

2013-12-15

How to Cite

Stupák, M., & Antalík, M. (2013). A Study of The Molten Globule State of Cytochrome C by Viscometry. Chemické Listy, 107(12), 969–972. Retrieved from http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/592

Issue

Vol. 107 No. 12 (2013)

Section

Articles